RCMI AT XAVIER
 
 
Header
 
Cell and Molecular Biology Core
seporator

Cell and Molecular Resources

BioRad Experion (Chip CE)

  • RNA separation and quality
  • DNA separation
  • Protein separation

Location: Qatar 411

http://www.xula.edu/rcmi/bio%20rad%20experion.jpg

http://www.xula.edu/rcmi/nano%20drop.jpg

Nano Drop Spectrophotometer

  • Low Vol Spectrophotometer (1 ul )
  • Scanning
  • Predefined methods for DNA, RNA, Protein Assays, etc.

Location: Qatar 411

Rotor-Gene Q

  • Unique rotary design Real Time PCR instrument
  • Eliminates sample-to-sample variation
  • Eliminates the need for reference dye
  • Wide range spanning UV to infrared wave length
  • Low 10 ul reaction volumes

Location: Qatar 411

http://www.xula.edu/rcmi/real%20time%20pcr.jpg

Real Time PCR: BioRad My IQ

  • PCR
  • Real Time PCR

Location: Qatar 411

Analytical Resources

http://www.xula.edu/rcmi/plate%20reader.jpg

Biotek Synergy 4 Plate Reader

  • Absorbance (Scanning and Fixed)
  • Fluorescence (Scanning and Fixed)
  • Fluorescence Polarization
  • Luminescence
  • Multi Plate Format

Location: Pharmacy Room 311

The Bio-Rad Molecular Imager Versadoc MP 4000 System
• A versatile and quantitative CCD based imaging system capable of supporting a wide array of detection methods, including fluorescence, chemiluminescence , densitometry, and chemiflourescence .
• Red, Green, and Blue illumination sources allow imaging of multiplexed fluorescent samples.
• Quantity One software can overlay images, which are acquired using various detection methods, into one image for analysis. Other available software packages, such as PDQuest software allow quantitative comparisons between images.
Location: Pharmacy rm 306

http://www.xula.edu/rcmi/versadoc.jpg

2D Proteomics Resources

http://www.xula.edu/rcmi/proteomics.jpg

  • Protein separation on 2D gel electrophoresis
  • Gel imaging on Versadoc
  • Gel analysis using Quantity Two software
  • Spot excision
  • In-gel digestion with trypsin
  • Final sample preparation for HPLC-MS/MS identification of proteins.

Cell Culture

  • Maintenance of cell stocks
  • Bulk Culture of Cells
  • Plating of cells
  • Media formulation
  • Cryo preservation of cell stocks
  • Transfection of plated cells
  • Creation of stably transfected cell lines
  • Cell based assays in plates or flasks
    • Proliferation
    • Reporter gene
    • Toxicity

http://www.xula.edu/rcmi/cell%20culture.jpg

 

 
Campus Map        Directory         Contact Us         EMERGENCY PREPAREDNESS    © Xavier University of Louisiana. All rights reserved.
(504) 486-7411
EST 1925