Cell and Molecular Biology Core

Cell and Molecular Resources

BioRad Experion (Chip CE)

  • RNA separation and quality
  • DNA separation
  • Protein separation

Location: Qatar 411



Nano Drop Spectrophotometer

  • Low Vol Spectrophotometer (1 ul )
  • Scanning
  • Predefined methods for DNA, RNA, Protein Assays, etc.

Location: Qatar 411

Rotor-Gene Q

  • Unique rotary design Real Time PCR instrument
  • Eliminates sample-to-sample variation
  • Eliminates the need for reference dye
  • Wide range spanning UV to infrared wave length
  • Low 10 ul reaction volumes

Location: Qatar 411


Real Time PCR: BioRad My IQ

  • PCR
  • Real Time PCR

Location: Qatar 411

Analytical Resources


Biotek Synergy 4 Plate Reader

  • Absorbance (Scanning and Fixed)
  • Fluorescence (Scanning and Fixed)
  • Fluorescence Polarization
  • Luminescence
  • Multi Plate Format

Location: Pharmacy Room 311

The Bio-Rad Molecular Imager Versadoc MP 4000 System
• A versatile and quantitative CCD based imaging system capable of supporting a wide array of detection methods, including fluorescence, chemiluminescence , densitometry, and chemiflourescence .
• Red, Green, and Blue illumination sources allow imaging of multiplexed fluorescent samples.
• Quantity One software can overlay images, which are acquired using various detection methods, into one image for analysis. Other available software packages, such as PDQuest software allow quantitative comparisons between images.
Location: Pharmacy rm 306


2D Proteomics Resources


  • Protein separation on 2D gel electrophoresis
  • Gel imaging on Versadoc
  • Gel analysis using Quantity Two software
  • Spot excision
  • In-gel digestion with trypsin
  • Final sample preparation for HPLC-MS/MS identification of proteins.

Cell Culture

  • Maintenance of cell stocks
  • Bulk Culture of Cells
  • Plating of cells
  • Media formulation
  • Cryo preservation of cell stocks
  • Transfection of plated cells
  • Creation of stably transfected cell lines
  • Cell based assays in plates or flasks
    • Proliferation
    • Reporter gene
    • Toxicity



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